Specifications

Vessel types

Vessel types:

0.3

0.4

Laboratory Fermentor-Bioreactor 0.3 l vessel

Laboratory Fermentor-Bioreactor 0.4 l vessel

Laboratory Fermentor-Bioreactor 1 l vessel

Laboratory Fermentor-Bioreactor 3 l vessel

Laboratory Fermentor-Bioreactor 7 l vessel

Volume [L]: ^*)

0.035–0.4

0.15–0.45

0.3–1.7

0.5–3

1–6

Height [cm]: ^**)

Diameter [cm]: ^**)

^*) Volume - is apx. minimal and maximal working volume. May vary according to conditions of each application.

^**)Height + Diameter - apx. dimensions of the vessel for autoclave with typical accessories. May vary according to conditions of each application.

Bioreactor Technical Specification

The LAMBDA MINIFOR fermentor/bioreactor is controlled by two microprocessors.

Parameter	Description
Power	Universal power supply for mains 100-245 V AC/50-60Hz, 560W, CE conform
Dimensions	22 x 40 x 38 cm (W x D x H)
Display	LCD 4 x 40 digits with backlight illumination
Fermentor vessel	Pyrex glass with 6 to 8 side necks; 0.3, 0.4, 1, 3, 7 liter vessels
Temperature control	High efficiency 150 W infrared (IR) radiation heat source with gilded parabolic reflector
Regulation	from 5°C over RT to 70°C
Measurement	from 0 to 99.9°C in 0.1°C steps
Precision	+/- 0.2°C (0 to 60°C)
Sensor	Pt 100 incorporated in the glass electrode of the pH probe
pH control	sterilisable pH electrode pH 0-14 with automatic temperature correction, two-point semiautomatic calibration and Variopin connector
Resolution	0.01 pH unit
Precision	+/- 0.02 pH unit
pO2 control	sterilisable Clark type oxygen sensor with fast response, automatic temperature correction, two-point semiautomatic calibration, dissolved oxygen (DO) control through regulation of the airflow rate
Range	0 to 25 mg oxygen/ l, in 0.1 mg/l steps
Air flow	0 to 5 l/min in 0.01 l/min steps, measured by precise mass flow meter, linearity +/- 3%, reproducibility +/- 0.5%
Control	proportional valve controlled by microprocessor
For supplied air pressure	0.05 – 0.2 MPa (0.5 - 2 atm)
Agitation	50 W Vibromixer 0 to 20 Hz (0 to 1200 rpm) in 0.1 Hz steps (6 rpm) with 1 or more stirring discs; Sterility similar to magnetic coupling
Selectable parameter	an additional parameter can be controlled by the instrument (foaming control, weight (for continuous cultures), pCO₂, redox potential, conductivity, optical density, etc.); with standard 0-10V or 0-20mA output
Ports	One large quadruple sampling or additions port with four needles with LAMBDA PEEK double-seal connections, used for sampling, inoculation, antifoam, feeds, harvest, addition of correction solutions etc., additional double ports are available.
Pumps	up to 4 independent pumps (PRECIFLOW, MULTIFLOW, HIFLOW or MAXIFLOW) with speed variation from 0 to 100 % can be used with MINIFOR lab fermenter-bioreactor
Gas flow control	In addition to pumps, several electronic flow controllers with flow rate ranges of 0-5 l/min (MASSFLOW 5000) or 0-500 ml/min (MASSFLOW 500) can be used for the controlled addition of gases (e.g. N₂, O₂, air, CO₂) in cell cultures; freely configurable gas station module
Working temperature	0 – 40 °C
Working humidity	0 - 90 % RH, not condensing
Weight	7.5 kg
PC control	complete PC control and data processing using the fermentation software FNet (for up to 6 MINIFOR fermenters) or SIAM (for an even higher number of instruments)

Parameter

Description

Power

Universal power supply for mains 100-245 V AC/50-60Hz, 560W, CE conform

Dimensions

22 x 40 x 38 cm (W x D x H)

Display

LCD 4 x 40 digits with backlight illumination

Fermentor vessel

Pyrex glass with 6 to 8 side necks; 0.3, 0.4, 1, 3, 7 liter vessels

Temperature control

High efficiency 150 W infrared (IR) radiation heat source with gilded parabolic reflector

Regulation

from 5°C over RT to 70°C

Measurement

from 0 to 99.9°C in 0.1°C steps

Precision

+/- 0.2°C (0 to 60°C)

Sensor

Pt 100 incorporated in the glass electrode of the pH probe

pH control

sterilisable pH electrode pH 0-14 with automatic temperature correction, two-point semiautomatic calibration and Variopin connector

Resolution

0.01 pH unit

Precision

+/- 0.02 pH unit

pO2 control

sterilisable Clark type oxygen sensor with fast response, automatic temperature correction, two-point semiautomatic calibration, dissolved oxygen (DO) control through regulation of the airflow rate

Range

0 to 25 mg oxygen/ l, in 0.1 mg/l steps

Air flow

0 to 5 l/min in 0.01 l/min steps, measured by precise mass flow meter, linearity +/- 3%, reproducibility +/- 0.5%

Control

proportional valve controlled by microprocessor

For supplied air pressure

0.05 – 0.2 MPa (0.5 - 2 atm)

Agitation

50 W Vibromixer 0 to 20 Hz (0 to 1200 rpm) in 0.1 Hz steps (6 rpm) with 1 or more stirring discs; Sterility similar to magnetic coupling

Selectable parameter

an additional parameter can be controlled by the instrument (foaming control, weight (for continuous cultures), pCO₂, redox potential, conductivity, optical density, etc.); with standard 0-10V or 0-20mA output

Ports

One large quadruple sampling or additions port with four needles with LAMBDA PEEK double-seal connections, used for sampling, inoculation, antifoam, feeds, harvest, addition of correction solutions etc., additional double ports are available.

Pumps

up to 4 independent pumps (PRECIFLOW, MULTIFLOW, HIFLOW or MAXIFLOW) with speed variation from 0 to 100 % can be used with MINIFOR lab fermenter-bioreactor

Gas flow control

In addition to pumps, several electronic flow controllers with flow rate ranges of 0-5 l/min (MASSFLOW 5000) or 0-500 ml/min (MASSFLOW 500) can be used for the controlled addition of gases (e.g. N₂, O₂, air, CO₂) in cell cultures; freely configurable gas station module

Working temperature

0 – 40 °C

Working humidity

0 - 90 % RH, not condensing

Weight

7.5 kg

PC control

complete PC control and data processing using the fermentation software FNet (for up to 6 MINIFOR fermenters) or SIAM (for an even higher number of instruments)