MINI-4-GAS - Automatic gas mixing

MINI-4-GAS station: Automatic gas-mixing module

What is it about?

The MINIFOR bioreactor and fermentor system is used for growing and testing bacteria, yeast, fungi, mammalian cells and stem cells.

MINI-4-GAS is a module for MINIFOR that allows a completely automatic gas-mix.

The MINI-4-GAS consists of MASSFLOWs and PC-Software.

MASSFLOWS will be measuring and regulating the gas-flow of the desired gasses.

It could be controlled remotely using LAMBDA SIAM PC control software extension.

LAMBDA MASSFLOW

Which gases do your cells need?

Example 4-GAS Mixing for mammalian cells: pO2 control & O2 enrichment pH control by CO2 Constant gas flow

Build now your own automatic gas-mixing module!

MINI-4-GAS is as easy to interlock as plastic bricks:

The bricks of MINI-4-GAS are LAMBDA MASSFLOWS. Each MASSFLOW will control one gas in your gas-mixture. You can choose two, three or four gases for your gas-mix.

The MASSFLOWS will dose and measure the gas flow rates accoring your set-points (flow rate / pH / pO₂ / pCO₂…)

The MINI-4-GAS software (a SIAM extension) is the brain of the gas-mix. The PC-software controls, visualizes and saves all gas-flow data.

The MINIFOR bioreactor dissolve the gases by micro-sparger, surface-aeration and excellent stirring with FISH-TAIL – a most efficient gas-uptake under minimized shearing forces.

The PC-Software

The SIAM extension

Real-time trend graphs Vizualization of the entire process & data storage.
Individual PID controller for each gas Easy set-point adjustment Low & High flow rate limits for each gas
pO2 control with Air & Oxygen (O2-enrichment)  pH control with Carbon dioxide Optional: constant total gas flow (exclusive flow)

LAMBDA ADVANTAGES

• Maintains optimal physiologic culture conditions

• Optimized process efficiency

• Retain reproducible production

• Preclude limiting conditions

• Cell line stability

• Prevent inducing physical & chemical stress

• Increase titer / product yield

• Satisfy the cells requirement

• Grow high-density cultures

• Optimized substrate conversion

Compared to most bio-transformations with quick growing yeast or bacteria, mammalian cell cultures by their low specific growth rates have to be cultured for weeks or even months for satisfying the product yields.

MINIFOR bioreactor offers you the “Lambda Easy Sterility” concept and an excellent peristaltic pumps specially  constructed for the long-term cell cultures. The MINIFOR with the 4-gas-mixing-module maintains the optimal physiologic conditions for your mammalian cells and the stem cell cultures using the  modern control strategies.

Controlling the critical process parameters will help you to optimize the growth rates as well as to achieve the higher titres during protein processing and hormone production.